ORIGINAL ARTICLE
Year : 2008 | Volume
: 7 | Issue : 4 | Page : 163--167
Electropherotypes and subgroups of group A rotaviruses circulating among diarrhoeic children in Kano, Nigeria
AA Dzikwi1, JU Umoh1, JKP Kwaga1, AA Ahmad2, M deBeer3, AD Steele3
1 Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria, Nigeria
2 Department of Microbiology, Faculty of Science, Ahmadu Bello University, Zaria, Nigeria
3 MRC/MEDUNSA Diarrheal Pathogens Research Unit, Medical University of South Africa, Pretoria, South Africa
Correspondence Address:
A A Dzikwi
Department of Veterinary Public Health and Preventive Medicine, Ahmadu Bello University, Zaria
Nigeria
Background: It is estimated that about 600 000 children die annually as a result of severe dehydrating diarrhea caused by rotaviruses. The virus is a double stranded RNA (dsRNA) virus with 11 segments. Group A rotaviruses show a characteristic 4-2-3-2 pattern following electrophoresis. The VP6 subgroups, I and II exist. This work was carried out to study the prevalence of rotavirus infection among children 0-5 years with diarrhea in Kano, and to determine the circulating subgroups and electropherotypes and of the rotavirus isolates.
Methods: Two hundred and eighteen stool specimens from children 0-60 months (198 diarrheic and 20 non-diarrheic) were collected from different hospitals and health care centers in Kano and subjected to group A rotavirus enzyme linked immunosorbent assay (ELISA) to determine presence of group A rotavirus, subgroup ELISA to determine the VP6 subgroups and polyacrylamide gel electrophoresis (PAGE) to determine the electropherotypes present.
Results: The long electropherotypes (47.05%) of four variations dominated over the short electropherotype (17.64%). About 11.76% of the isolates were of mixed infection. Dominance of subgroup II (45%) over subgroup I (25%), and the presence of both subgroups I and II (10%) and neither subgroup I nor II (15%) was observed in this study.
Conclusion: Information on the genomic diversity of the RNA electropherotypes in this region, Kano, is reported in this study.
How to cite this article:
Dzikwi A A, Umoh J U, Kwaga J, Ahmad A A, deBeer M, Steele A D. Electropherotypes and subgroups of group A rotaviruses circulating among diarrhoeic children in Kano, Nigeria
.Ann Afr Med 2008;7:163-167
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How to cite this URL:
Dzikwi A A, Umoh J U, Kwaga J, Ahmad A A, deBeer M, Steele A D. Electropherotypes and subgroups of group A rotaviruses circulating among diarrhoeic children in Kano, Nigeria
. Ann Afr Med [serial online] 2008 [cited 2022 May 24 ];7:163-167
Available from: https://www.annalsafrmed.org/article.asp?issn=1596-3519;year=2008;volume=7;issue=4;spage=163;epage=167;aulast=Dzikwi;type=0 |
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