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An in vitro evaluation of efficacy of ViaSpan, aloe vera, gatorade solution, and propolis storage media for maintaining the periodontal ligament cell viability
Rajat Misurya1, Sandeep Sharma2, Prabu Mahin Syed Ismail3, Nitika Gupta4, Reshma Rajan5, Rasveen Kaur6, Prashant Babaji7
1 Department of Dental Surgery, MLB Medical College, Jhansi, Utter Pradesh, India
2 Department of Oral Pathology, College of Medical Sciences - Dental Program, Bharatpur, Nepal, India
3 Department of Restorative Dentistry, College of Dentistry, at AL Russ, Qassim University, Kingdom of Saudi Arabia
4 Department of Prosthodontics, Maharishi Markandeshwar College of Dental Sciences and Research, Mullana, Haryana, India
5 Department of Pediatric and Preventive Dentistry, PSM College of Dental Science and Research, Akkikavu, Thrissur, Kerala, India
6 Department of Peridontology and Oral Implantology, Maharishi Markandeshwar College of Dental Sciences and Research, Mullana, Haryana, India
7 Department of Pediatric and Preventive Dentistry, Sharavathi Dental College, Shimoga, Karnataka, India
Correspondence Address:
Prashant Babaji
Department of Pediatric and Preventive Dentistry, Sharavathi Dental College, Shimoga, Karnataka
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/aam.aam_71_20
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Background: Replantation is a commonly performed method for avulsed tooth. A vital periodontal membrane (periodontal ligament [PDL]) is significant for the successful healing of replanted teeth. Hence, various storage media are used to preserve the viability of periodontal cells before replantation. Objectives: The present study was conducted to evaluate the efficacy of ViaSpan, Aloe vera, Gatorade solution, and propolis storage media for maintaining the PDL cell viability. Materials and Methods: The present study was conducted on 40 recently extracted teeth which were randomly divided into four study storage groups: Group I: ViaSpan, Group II: Aloe vera, Group III: Gatorade solution, and Group IV: Propolis. Later they were subjected to centrifugation, and the cells from supernatant were colored with 0.4% trypan blue for determination of viability. The obtained data were statistically evaluated with SPSS package (21.0 version, Inc.; Chicago, IL, USA) using analysis of variance, Mann–Whitney test, and Post hoc tests. Results: The mean viable periodontal cell in Group I was 30.2 cumm, in Group II was 24.6 cumm, Group III was 14.5 cumm, and Group IV in 31.4. The difference was significant (P < 0.01). Post hoc test between different groups revealed a significant difference in mean viable periodontal cells (P < 0.001). Propolis, ViaSpan, and Aloe vera had higher pH and osmolality values. Conclusion: This study found that propolis had higher periodontal cell viability followed by ViaSpan solution and Aloe vera and least in Gatorade solution. Propolis, ViaSpan, and Aloe vera media can be used as a storage media.
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